Changes in Cytokine Levels and Oxidative Status Against Coxiella burnetii Infection in Aborted Sheep

Authors : Serpil Aygörmez, Aliye Gülmez Sağlam, Elif Çelik, Şaban Maraşlı

Pages : 207-216

Doi:10.53518/mjavl.1718081

View : 77 | Download : 201

Publication Date : 2025-12-25

Article Type : Research Paper

Abstract :The aim of this study was to investigate the levels of nuclear factor kappa-B (NF-B), malondialdehyde (MDA), glutathione (GSH), interleukin 1β (IL-1β) and tumor necrosis factor-alpha (TNF-α) against naturally acquired Coxiella burnetii (C.burnetii) infection in abortive sheep. The sera of aborted (n = 20) and healthy (n = 20) sheep were used in the study. The enzyme-linked immunosorbent assay (ELISA) was used to detect host antibodies against C. burnetii. The MDA was higher in C. burnetii-positive sheep than in healthy group (p < 0.001). GSH level were significantly lower in C. burnetii-positive sheep compared with the healthy sheep. The cytokine levels were statistically higher in C. burnetii-positive sheep than in healthy sheep (p < 0.001). A positive correlation was found between MDA, NF-B, TNF-α, and IL-1β levels and a negative correlation between GSH level (p < 0.01). In addition, a strong negative correlation was observed between the levels of cytokines and antioxidants (p < 0.01). In conclusion, oxidative stress and cytokine markers may serve as valuable diagnostic and prognostic tools for C. burnetii infection in sheep.
Keywords : Abort, C. burnetii, sitokin, oksidatif stres, koyun

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