Determining the enterotoxin genes and methicillin resistance in Staphylococcus Aureus isolated from goat milk and its products

Authors : Mustafa BİLGİN, Mustafa ATASEVER

Pages : 147-154

Doi:10.51354/mjen.1185346

View : 20 | Download : 12

Publication Date : 2022-12-31

Article Type : Research Paper

Abstract :Milk and its products can be frequently contaminated with enterotoxigenic and methicillin-resistant S. aureus, and in such a case, it causes various diseases, especially staphylococcal food poisoning. In the present study, 100 sample materials insert ignore into journalissuearticles values(50 goat milk, 25 goat cream, and 25 goat cheese); were collected from 65 livestock farms in Erzurum. All samples were analyzed and tested selectively according to the EN ISO 6888-1 procedure standard. The obtained isolates were examined with the PCR in terms of nuc, Panton-Valentine Leukocidin insert ignore into journalissuearticles values(PVL);, mecA, and enterotoxin genes. S. aureus was detected in 4 of 50 insert ignore into journalissuearticles values(8%); milk samples, 2 of 25 insert ignore into journalissuearticles values(8%); cream samples, 3 of 25 insert ignore into journalissuearticles values(12%); cheese samples, and 9 of the total samples insert ignore into journalissuearticles values(9%);. While the incidence of methicillin resistance was found to be 3% by the disc diffusion method, the incidence of the mecA gene was found to be 2% by PCR. In terms of enterotoxin genes, 8% insert ignore into journalissuearticles values(4/50); of milk samples, 12% insert ignore into journalissuearticles values(3/25); of cheese samples, and 8% insert ignore into journalissuearticles values(2/25); of cream samples contained at least one enterotoxin gene. In total, 9% of 100 samples contained enterotoxigenic S. aureus. In conclusion, the consumption of enterotoxigenic and mecA positive S. aureus-containing raw milk, dairy products that have not been adequately heat-treated, or milk and products that are not kept in proper storage conditions pose a danger to public health.
Keywords : Goat milk, methicillin resistance, staphylococcal enterotoxins, Staphylococcus aureus