Combined polymerase chain reaction (pcr) and fluorescence in situ hybridization (fish) analysis of y chromosome loss related to age and disease status in prostate cancer

Authors : LN Türkeri, W Sark, SR Wolman, MA Micale, DJ Grignon, IJ Powell, JE Pontes, JA Macoska

Pages : 191-195

View : 13 | Download : 12

Publication Date : 1994-11-01

Article Type : Research Paper

Abstract :Aneusomy of the Y chromosome was examined in paired malignant and benign prostate tissue from 31 radical prostatectomy specimens to analyze the possible relationship between Y chromosome loss and disease status, and to define its relationship to the aging process. Tissues from men aged 45-55 years or 56-75 years at the time of prostate cancer diagnosis were examined for Y chromosome dosage using Polymerase Chain Reaction insert ignore into journalissuearticles values(PCR); and Fluorescence In Situ Hybridization insert ignore into journalissuearticles values(FISH); techniques. PCR analysis of malignant tissue demonstrated Yp sequence losses in 0 % insert ignore into journalissuearticles values(0/20); of men aged 45-55 years and 18% insert ignore into journalissuearticles values(2/11); of men aged 56-75 years; no losses were observed in benign epithelium from either age group. FISH analysis demonstrated Y chromosome loss in 5% insert ignore into journalissuearticles values(1/20); of malignant tumors, 14% insert ignore into journalissuearticles values(1/7); of prostatic intraepithelial neoplasia insert ignore into journalissuearticles values(PIN); lesions, and 0% insert ignore into journalissuearticles values(0/20); of benign glands in men aged 45-55 years, and in 18% insert ignore into journalissuearticles values(2/11); of malignant glands, 50% insert ignore into journalissuearticles values(1/2); of PIN lesions and 9% insert ignore into journalissuearticles values(1/11); of benign glands in men aged 56-75 years. Interestingly, focal loss of the Y chromosome involving multiple or single malignant glands was observed by FISH analysis in 25% insert ignore into journalissuearticles values(5/20); of men aged 45-55 years and 1/11 insert ignore into journalissuearticles values(9%); of men aged 56-75 years. These results indicate that Y chromosome loss is associated with both the aging process and malignancy in the prostate.
Keywords : Prostate Neoplasms, Y Chromosome, In Situ Hybridization, Polymerase Chain Reaction