Tnf-a, scd8 and sll-2r levels in pbmc culture supernatants of children with pulmonary tuberculosis

Authors : I B Borlan, MM Başaran, S Bilsel, Y Toga

Pages : 136-139

View : 13 | Download : 6

Publication Date : 1995-07-01

Article Type : Research Paper

Abstract :This study was undertaken to investigate the impact of Mycobacterium tuberculosis insert ignore into journalissuearticles values(M. tuberculosis); infection on in-vitro production of tumor necrosis factor alpha insert ignore into journalissuearticles values(TNF-a);, soluble CD8 insert ignore into journalissuearticles values(sCD8); and soluble IL-2 receptor insert ignore into journalissuearticles values(slL-2R); by peripheral blood mononuclear cells insert ignore into journalissuearticles values(PBMCs); from children with pulmonary tuberculosis insert ignore into journalissuearticles values(TB); versus controls. PBMCs from 8 children with newly diagnosed pulmonary TB were stimulated with PHA and LPS either separately or in combination. Five healthy children and 4 adults were used as controls. Cell free supernatants were assayed for the presence of TNF-a, sCD8 and slL-2R with a sandwich enzyme- linked immunosorbent assay insert ignore into journalissuearticles values(ELISA);. Although the median of TNF-a production was significantly higher in the unstimulated supernatants of patients insert ignore into journalissuearticles values(42.5pg/ml, 20.0-320.0); when compared to controls insert ignore into journalissuearticles values(15pg/ml, 0-90.0); insert ignore into journalissuearticles values(p<0.05); determination of sCD8 and slL-2R levels revealed no significant difference between the patients and controls. Our findings indicated that PBMC obtained from children with TB spontaneously secreted high levels of TNF-a which may exert immunoregulatory effects and thus mediate some of the clinical manifestations of tuberculosis.
Keywords : TNF a, sCD8, SİL 2R, tuberculosis