2-deoxyglucose transport in human peripheral lymphocytes rat lymphocytes and thymocytes; effects of insulin on 2-deoxyglucose transport in these cells

Authors : G HERGENÇ, K EMERK

Pages : 23-28

View : 10 | Download : 7

Publication Date : 1996-01-01

Article Type : Research Paper

Abstract :Glucose transport into human peripheral lymphocytes, rat lymphocytes, and rat thymocytes were investigated using 2-deoxyglucose insert ignore into journalissuearticles values(2-DOG); in the transport studies. Our results showed rat thymocytes to have a high affinity and a low affinity system for transporting 2-DOG with Km values of 0.11 mM and 7.14 mM respectively. Both of the systems were inhibited by phloretin insert ignore into journalissuearticles values(0.1 mM);, a specific inhibitor of carrier mediated transport. Human peripheral lymphocytes and rat lymphoctes transported 2-DOG with Km values of 5.26 mM and 3.57 mM respectively. While insulin had no effect on this transport in thymocytes and lymphocytes, it inhibited the efflux of 2-DOG from the cells.
Keywords : 2 DOG, uptake, lymphocytes, thymocytes, transport, insulin