Recent advances in male factor infertility and intracytoplasmic sperm injection

Authors : M A Aleman, M Erenus, F Durmuşoğlu

Pages : 154-157

View : 10 | Download : 9

Publication Date : 1997-07-01

Article Type : Research Paper

Abstract :The first trial of recovering a preimplantation stage embryo from flushing of a rabbit oviduct and transferring the embryo to a foster mother was demonstrated by Walter Heape in 1890 insert ignore into journalissuearticles values(1);. However, Chang et al insert ignore into journalissuearticles values(2); in 1959 reported the first successful fertilization of rabbit eggs in vitro. The first successful human pregnancy and birth after in vitro fertilization insert ignore into journalissuearticles values(IVF); was reported by Steptoe and Edwards in 1978 insert ignore into journalissuearticles values(3);. IVF by insemination has been used to treat couples with male factor infertility for over a decade with relatively disappointing results, presumably because the sperm cells were unable to penetrate the zona pellucida and fuse with the oolemma. This has led to the application of gamete micromanipulation. The initial approach to assisted fertilization was to partially bypass the zona pellucida. An animal model developed by Gordon and Talansky insert ignore into journalissuearticles values(4); demonstrated that an artificial opening introduced in the zona pellucida before insemination enhanced fertilization. Partial zona dissection insert ignore into journalissuearticles values(PZD); involved mechanical opening of the zona pellucida by a narrow microneedle while the oocyte was suspended in a hyperosmatic solution. Unfortunately this technique has not been successful. Fertilization rates were very low and there was a relatively high incidence of polyspermy insert ignore into journalissuearticles values(the penetration of more than one spermatozoan);.
Keywords : Recent advances in male factor infertility and intracytoplasmic sperm injection