MOLECULAR EPIDEMIOLOGY OF MRSA ISOLATES IN THE INTENSIVE CARE UNIT DURING A 4-YEAR-PERIOD

Authors : Burak Aksu, Ayşegül Yağcı, Arzu İlki, Güner Söyletir

Pages : 151-154

View : 17 | Download : 12

Publication Date : 2016-12-03

Article Type : Research Paper

Abstract :Objective: Methicillin resistant Staphylococcus aureus insert ignore into journalissuearticles values(MRSA); is a common cause of nosocomial infections in hospitalized patients , particularly in those who stay in intensive care unitinsert ignore into journalissuearticles values(ICU);. We aimed to compare MRSA strains isolated from ICU in order to detect relatedness between them since accurate epidemiological typing by reproducible and rapid methods is a major step in determining MRSA clones and sources of transmission for immediate infection control. Methods: From February 1998 to November 2001, 56 MRSA Isolates from various clinical specimens from different patients who were hospitalized in ICU of Marmara University Hospital were included in the study. These isolates were identified as MRSA by standard methods. Genotyping analysis was done by AP- PCRinsert ignore into journalissuearticles values(arbitrarily primed polymerase chain reaction);. Results and conclusion: The specimens which the MRSA strains isolated were as follows: 38insert ignore into journalissuearticles values(67.8%); from respiratory tractinsert ignore into journalissuearticles values(deep tracheal aspirate and sputum);, 10insert ignore into journalissuearticles values(17.8%); from blood, 4insert ignore into journalissuearticles values(7.1%); from wound infection and abscess, 2insert ignore into journalissuearticles values(3.6%);from catheters, 1insert ignore into journalissuearticles values(1.8%); from urine sample and 1insert ignore into journalissuearticles values(1.8%); from joint fluid. By AP-PCR analysis 28/56 isolates exhibited 11 incidences of clusters throughout the study period. The longest time for a given incidence was 3 months and a given incidence affected 4 patients at most. We conclude that the AP-PCR method can easily be used in order to evaluate genotypic relatedness between MRSA isolates in our institution. Key Words: Methicilline resistant Staphylococcus aureus, Intensive care unit, Arbitrarily primed polymerase chain reaction
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