INHIBITION OF NITRIC OXIDE SYNTHESIS AMELIORATES BURN-INDUCED REMOTE ORGAN INJURY IN RATS: A LIGHT MICROSCOPIC STUDY

Authors : Özlem Güneysel, Berna Oktar, Berrak Yeğen, Cumhur Yeğen, Serap Arbak

Pages : 155-160

View : 12 | Download : 6

Publication Date : 2016-12-03

Article Type : Research Paper

Abstract :Objective: To investigate the role of endogenous nitric oxide insert ignore into journalissuearticles values(NO); on remote organ injury in the early phase of burn trauma. Methods: Wistar albino rats insert ignore into journalissuearticles values(200-300 g); were exposed to 90°C insert ignore into journalissuearticles values(burn); or 25°C insert ignore into journalissuearticles values(sham); water bath for 10 sec. Results: Microscopic score in the stomach was increased in the burn group compared to sham. Hemorrhage areas, epithelial desquamation and glandular cell degeneration were observed in the gastric mucosa of the burn group. NG-nitro-L- arginine methyl ester insert ignore into journalissuearticles values(L-NAME); treatment reduced the burn-induced damage score with an intact glandular architecture. In NG-nitro-D- arginine methyl ester insert ignore into journalissuearticles values(D-NAME); pretreated groups, histologic scores were not different than burn group, with desquamated and degenerated surface epithelium. L-arginine insert ignore into journalissuearticles values(L-arg); plus L- NAME pretreatment partially reversed this effect with prominent gastric mucosal damage. In the liver, the microscopic score was increased in the burn group compared to sham. Hepatocyte vacuolar degeneration, sinusoidal congestion and increased number of Kupffer cells were observed in the burn group. Hepatic injury was slightly attenuated by L-NAME treatment whereas D-NAME or L-arg plus L-NAME pretreatment was ineffective. Conclusion: In conclusion, inhibition of NO synthesis ameliorates gastric and hepatic damage, emphasizing the critical role of NO in the burn-induced remote organ injury. Key Words: Burns, Nitric oxide, Remote organ injury, Myeloperoxidase activity
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