THE EFFECT OF α-MELANOCYTE STIMULATING HORMONE ON BURN-INDUCED OXIDANT PRODUCTION BY RAT PERITONEAL NEUTROPHILS

Authors : Berna OKTAR, Meral YÜKSEL, İnci ALİCAN

Pages : 7-11

View : 26 | Download : 18

Publication Date : 2016-12-03

Article Type : Research Paper

Abstract :Objective:  Previous findings related to the anti-inflammatory effects of α-melanocyte stimulating hormone insert ignore into journalissuearticles values(α-MSH); indicate that the peptide might inhibit inflammation by antagonizing the effects of local cytokines or by inhibiting neutrophil functions. In addition, it is a potent inhibitor of the induction of the inducible nitric oxide synthase insert ignore into journalissuearticles values(iNOS); in cultured macrophages and of nitric oxide insert ignore into journalissuearticles values(NO); production in a sepsis model. This study was designed to investigate whether α-MSH affects reactive oxygen metabolite production by rat peritoneal neutrophils following burn injury and whether NO is involved in this effect. Methods:  The neutrophils were removed from the peritoneum of the rats 6 h after burn or sham trauma. Cells insert ignore into journalissuearticles values(2 X 10 6  /ml); were treated with the increasing concentrations of α-MSH insert ignore into journalissuearticles values(10 –12  M, 10 –10  M, 10 –8  M or 10 –6  M);. Nitric oxide donor, sodium nitroprusside ranging from insert ignore into journalissuearticles values(10 –10  - 10 –4  M); was added to the cells in the presence of α-MSH. Reactive oxygen metabolite production was measured by chemiluminescence insert ignore into journalissuearticles values(CL); technique using luminol or lucigenin probes. Results:  Lucigenin CL value of cells obtained from burn animals was significantly higher than those obtained from the sham group. α-MSH caused significant reductions in high lucigenin CL values and this effect was partly inhibited by sodium nitroprusside. Conclusion:  Our results suggest that α-MSH is effective in preventing oxidant production by neutrophils activated by burn trauma, at least in part, by a mechanism involving nitric oxide. 
Keywords : α melanocyte stimulating hormone, Burn, Reactive oxygen species, Nitric oxide, Chemiluminescence