Antioxidant and antimicrobial activity of Ficus sycomorus fruit and leaf extracts

Authors : Melis SUMENGEN OZDENEFE, Feryal TANOGLU, Kaya SUER, Emrah GULER, Hatice Aysun MERCIMEK TAKCI

Pages : 133-140

Doi:10.5472/marumj.816301

View : 20 | Download : 8

Publication Date : 2020-10-23

Article Type : Research Paper

Abstract :Objective: The aim of this study was to determine the antioxidant and antimicrobial activity of Ficus sycomorus fruit and leaf extracts obtained from the Turkish Republic of Northern Cyprus. Materials and Methods: Fruits and leaves of F. sycomorus were collected from the Kyrenia region of Northern Cyprus in July 2018. The leaf and fruit samples were extracted with the distilled water, methanol, ethanol, acetone and chloroform solvents insert ignore into journalissuearticles values(1:10 [w/v]);. After evaporation, samples were suspended in methanol at the final concentration of 100 mg/mL. The antimicrobial activity of the leaf and fruit extracts was evaluated using the Kirby-Bauer Disk Diffusion Method. Total phenolic content insert ignore into journalissuearticles values(TPC); and total flavonoid content insert ignore into journalissuearticles values(TFC); of the extracts were determined using the methods reported by Stankovic in 2011 and Sharm and Vig in 2013. Antioxidant activity of samples was tested using free radical scavenger method. Results: The leaf extracts of F. sycomorus was active. The inhibition zone diameters ranged from 1.8mm to 13.00 mm. Fruit extracts and methanol controls showed no inhibitory effect on strains. However, bacteriostatic activity against Enterococcus faecalis was observed in fruit-water extract. The highest antimicrobial activity was shown against Staphylococcus aureus insert ignore into journalissuearticles values(13 mm); for ethanolic extracts at 100 mg/mL concentration. Minimal inhibition concentration insert ignore into journalissuearticles values(MIC); for ethanolic extract was observed starting at 25 mg/mL concentration against S. aureus. Although, no antimicrobial activiy was observed in fruit extracts, the highest 2,2-diphenyl 1-picrylhydrazyl insert ignore into journalissuearticles values(DPPH); activiy and phytochemical content were recorded in fruit extracts. Conclusion: These results demonstrate that leaf extracts of F. sycomorus can be used as a curative agent for the treatment of S. aureus and fungal infections and may be effective against pathogenic microorganisms that are resistant to antibiotics. Antioxidant content of fruit and leaf extracts can be effective against the negative effects of free radicals.
Keywords : Antioxidant activity, F sycomorus, S aureus