The effects of alpha-lipoic acid (ALA) on the urinary bladder injury in rats exposed to chronic stress: A histochemical study

Authors : Necati YURDAKUL, Esra CIKLER, Hale Zerrin TOKLU, Serap SIRVANCI

Pages : 263-269

Doi:10.5472/marumj.1191211

View : 13 | Download : 6

Publication Date : 2022-10-31

Article Type : Research Paper

Abstract :Objective: In the present study, we aimed to investigate the morphological and biochemical effects of alpha-lipoic acid insert ignore into journalissuearticles values(ALA); on bladder injury caused by water avoidance stress insert ignore into journalissuearticles values(WAS); and to show its effect on the number of degranulated mast cells, which increase after stress. Materials and Methods: Wistar albino rats were subjected to WAS and the animals in the treatment group were injected ALA. After the urinary bladder tissues were subjected to routine tissue processing, hematoxylin-eosin staining and periodic acid-Schiff reaction were applied to observe general morphology and acidic toluidine blue method to investigate mast cells. Biochemical assessments of malondialdehyde insert ignore into journalissuearticles values(MDA); and glutathione insert ignore into journalissuearticles values(GSH); were also obtained. Transmission electron microscope was used for the ultrastructural, and scanning electron microscope for the topographical analyses. Results: The experiments showed that chronic stress caused injury in the bladder, increased degranulated and total number of mast cells and decreased GSH and increased MDA levels. ALA treatment after WAS ameliorated bladder injury in most areas, decreased degranulated and total mast cell number and increased GSH and decreased MDA levels. Conclusion: It was concluded that ALA can be a useful agent in the treatment of interstitial cystitis.
Keywords : Alpha lipoic acid ALA, Interstitial cystitis, Mast cell, Stress, Urinary bladder