Morphological and biochemical evaluation of effects of Myrtus communis L. extract on heart and aorta in high fat-diet-induced obese rats

Authors : Nagehan OZYILMAZ YAY, Nurdan BULBUL AYCI, Rumeysa KELES KAYA, Ali SEN, Goksel SENER, Feriha ERCAN

Pages : 162-170

Doi:10.5472/marumj.1302544

View : 52 | Download : 147

Publication Date : 2023-05-31

Article Type : Research Paper

Abstract :Objective: The purpose of this study was to examine the protective effects of Myrtus communis L. insert ignore into journalissuearticles values(MC); extract on high fat-diet insert ignore into journalissuearticles values(HFD); induced heart and aorta damage by evaluating oxidative stress and the endothelial nitric oxide system insert ignore into journalissuearticles values(eNOS);. Materials and Methods: Wistar albino male rats were divided into 3 groups insert ignore into journalissuearticles values(n=7); as control, HFD, and HFD+MC. Rats in HFD and HFD+MC groups were HFD fed for 16 weeks and in the last 4 weeks saline or MC insert ignore into journalissuearticles values(100 mg/kg); was administered orally insert ignore into journalissuearticles values(5 days/week);. Triglyceride, cholesterol, and high-density lipoprotein insert ignore into journalissuearticles values(HDL); were estimated in blood serum. Tissue oxidative stress and inflammatory parameters were evaluated biochemically. Tissue morphologies, eNOS, inducible NOS insert ignore into journalissuearticles values(iNOS);, and NADPH oxidase-2 insert ignore into journalissuearticles values(NOX-2);-immunopositive and apoptotic cells were evaluated histologically. Results: Altered serum lipid profiles, degenerated heart, and aorta morphology, increased malondialdehyde, 8‐hydroxy‐2‐ deoxyguanosine, tumor necrosis factor-alpha, monocyte chemoattractant protein-1 and myeloperoxidase levels, and iNOS, NOX-2 immunopositive and apoptotic cells, decreased NO levels, eNOS-immunopositive cells in both tissues were observed in HFD group. All these parameters improved in the HFD+MC group. Conclusion: This study revealed that HFD-induced obesity increased iNOS activation and oxidative stress in the cardiac and aortic tissues of the rats. MC improved oxidant/antioxidant balance and prevented heart and aorta damage via eNOS involvement.
Keywords : High fat diet, Obesity, Myrtus communis L extract, Heart, Aorta