Investigation of Resistance and Clonal Relatedness Among The Nosocomial Acinetobacter Isolates

Authors : Ahmet ÇALIŞKAN, Rıza DURMAZ, Canan ATEŞ GÜRSOY, Nilay ILDIZ

Pages : 120-132

Doi:10.19127/mbsjohs.581706

View : 63 | Download : 16

Publication Date : 2019-08-28

Article Type : Research Paper

Abstract :Objective: This study focused on Acinetobacter spp. that were isolated from inpatients to determine the resistance rates to antibiotics and to monitor the resistance increase over the years. Method: To determine whether there was a clonal relationship between the strains using pulsed field gel electrophoresis insert ignore into journalissuearticles values(PFGE);, to evaluate the epidemiological and clinical data of patients, and to determine the factors that may be effective in the formation and spread of infection, antibiotic susceptibilities of Acinetobacter strains were evaluated by the Kirby Bauer disk diffusion method. Molecular typing was studied with PFGE. Results: Netilmicin had the least resistance insert ignore into journalissuearticles values(7%); among the strains, while the highest resistance was found against piperacillin insert ignore into journalissuearticles values(92%);, ceftriaxone insert ignore into journalissuearticles values(81%);, and doxycycline insert ignore into journalissuearticles values(69%);. When resistance profiles of strains were compared according to year insert ignore into journalissuearticles values(2005-2007);, no significant changes were found for resistance rates to piperacillin, ceftriaxone, gentamicin, doxycycline, and trimethoprim / sulfamethoxazole. Of all the 131 tested Acinetobacter spp., 82 insert ignore into journalissuearticles values(62.6%); of the strains were clustered with the PFGE method and 72.3% of the strains were clonally related. The increase in MDR, XDR and PDR rates among the isolated Acinetobacter spp. is remarkable. It was determined that the degree of transmission of the strains was quite high among patients. The clone in the hospital was able to remain in the environment for long periods and the general patient mortality rates were higher than other studies. Conclusions: According to results and with the support of molecular typing studies, the need for more effective prevention and control measures in our hospital was demonstrated.
Keywords : Acinetobacter, PFGE, cluster, resistant

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