A New Adapted Method For Screenıng Phaseolus Genotypes For Tolerance To Low Temperature

Authors : Kadhum Eman MUSS, Stoeva NEVENA, Nidal SHABAN

Pages : 45-49

View : 20 | Download : 19

Publication Date : 2010-06-25

Article Type : Research Paper

Abstract :The selection and characterization of 620 bean forms, from the genbank- Sadovo- Bulgaria, from ssp. volubillis & ssp. nanus was achieved. The studied genotypes are from Europe, North and South America, Africa & Asia. The study aimed to indicate the cool tolerant forms by using a new and original screening method. The photosynthetic intensity/through 14 CO2 /the activity of catalaze /gasometerically/ and peroxidase /colorimetricaly/ and the plastid pigment contents/ spectrophotometrically, of different genotypes snap bean with varied cool tolerance was studied. The genotypes which studied have widely differed in the peroxidase activity. The genotypes with highest cool tolerance are char-acterized with lower peroxidase activity, while the genotypes with moderate tolerance genotypes are characterized with increased enzyme activity- 588, 667, 845 E60s/g respectively. With the genotypes which have higher cool tolerance there is a tendency toward to increased plastid contents and catalase activity, but the differences are not so significant. The conclusion is that the peroxidase can be used as cool tolerance criterion.
Keywords : Fasulye genotipleri, soğuk toleransı, tarama metodu, plastid içeriği, peroksidaz aktivitesi, kata laz aktivitesi