Prenatal stress impairs recognition memory and leads to neurodevelopmental deficits in hippocampus of adolescent rats with early acute pentylenetetrazole-kindling

Authors : Kübra ÇELİK, Petek BİLİM, Gurur GARİP, Burak DURMAZ, Eser Y SÖZMEN, Meral BAKA

Pages : 340-347

Doi:10.18621/eurj.801699

View : 27 | Download : 13

Publication Date : 2021-07-04

Article Type : Research Paper

Abstract :Objectives: This study aimed to investigate the effects of prenatal stress insert ignore into journalissuearticles values(PS); on hippocampus of early acute pentylenetetrazole insert ignore into journalissuearticles values(PTZ);-kindled offspring in adolescence. Recognition memory, morphological changes and synaptophysin levels in hippocampus were evaluated. Methods: Restraint stress was induced to a group of pregnant dams and non-stressed insert ignore into journalissuearticles values(NA); group remained undisturbed. Next, male and female offspring were divided as 1. PS-PTZ, 2. PS -control, 3. NA-PTZ and 4. NA-control insert ignore into journalissuearticles values(n = 12 in each group);. The object recognition test was performed following PTZ injection insert ignore into journalissuearticles values(45 mg/kg); on postnatal day 10 insert ignore into journalissuearticles values(P10);. Brains were collected on postnatal day 35 insert ignore into journalissuearticles values(P35); to determine neuronal density and synaptophysin expression by immuno/-histological studies. Further, oxidative stress products in hippocampus were analyzed with different biochemical assays. Results: PS impaired recognition memory in PTZ group significantly insert ignore into journalissuearticles values( p = 0.03);; however, the impairment of PS was reversible in control group compared to PTZ insert ignore into journalissuearticles values( p = 0.04);. Furthermore, PS caused neuronal loss in CA1 insert ignore into journalissuearticles values( p = 0.01); and decreased synaptophysin expression in the CA3 area of hippocampus in PTZ group insert ignore into journalissuearticles values( p = 0.03);. PS also increased the oxidative stress markers in PTZ group significantly insert ignore into journalissuearticles values( p < 0.05);. Conclusions: These results suggest that PS causes neurodevelopmental deficits in adolescent hippocampus and recognition memory after early-life seizures prominently. However, the damage of only PS in adolescence can be reversible. Therefore, the effects of PS in the adult hippocampus and other regions of brain need to be further studied.
Keywords : prenatal stress, hippocampus, PTZ kindling, seizure, recognition memory