Effect of caffeine supplementation during treadmill exercise on hippocampal genes expression levels in adolescent rats

Authors : Fatma BAKIR, Özge Selin ÇEVİK, Seval MÜSÜROĞLU KELOĞLAN, Leyla ŞAHİN

Pages : 348-358

Doi:10.18621/eurj.1241667

View : 37 | Download : 12

Publication Date : 2023-03-04

Article Type : Research Paper

Abstract :Objectives: The usage of caffeine, a psychostimulant that is included in many foods and drinks, is rising, especially among young people. Also, caffeine works as an ergogenic substance during exercise to improve performance, and strength. In this study, our aim was to investigate the effect of caffeine consumption and exercise on hippocampal learning and memory functions in early life. Methods: Postnatal 28 days old Wistar albino male rats insert ignore into journalissuearticles values(n = 28); were randomly divided into 4 groups; control group insert ignore into journalissuearticles values(C);, caffeine group insert ignore into journalissuearticles values(Cf);, exercise group insert ignore into journalissuearticles values(E);, caffeine+exercise group insert ignore into journalissuearticles values(CfE);. Caffeine was dissolved in drinking water insert ignore into journalissuearticles values(0.3 g/L); and the treadmill exercise was applied 3 days a week. Following, the rats were applied to Morris Water Maze Test insert ignore into journalissuearticles values(MWMT); and open field test. N-methyl-D-aspartate insert ignore into journalissuearticles values(NMDA); receptors NR2A, NR2B, and brain-derived neurotrophic factor insert ignore into journalissuearticles values(BDNF); gene expression levels were investigated in hippocampus tissue by RT-PCR. Results: In MWMT, there was no significant difference in terms of learning and memory functions and hippocampal gene expression levels of the groups insert ignore into journalissuearticles values( p > 0.05);. In the open field test, the time spent in the center was decreased in the CfE group, and the number of entries to the center was decreased in the E and CfE groups compared to the control group insert ignore into journalissuearticles values( p < 0.05);. Conclusions: We assumed that caffeine given with exercise application caused anxiety behavior but did not affect learning and memory. There is a need for new studies investigating the effect of caffeine on exercise with different doses and durations depending on age.
Keywords : Caffeine, exercise, hippocampus, learning, memory

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