Protective effects of methylprednisolone in kidney: aortic occlusion-reperfusion model in rats

Authors : Serkan SEÇİCİ, Kadir Kaan ÖZSİN, Mözgür ÖZYİĞİT, Omer ARDA, Yasemin ÜSTÜNDAĞ

Pages : 567-573

Doi:10.18621/eurj.1181742

View : 12 | Download : 10

Publication Date : 2023-05-04

Article Type : Research Paper

Abstract :Objectives: Ischemia/reperfusion insert ignore into journalissuearticles values(I/R); injury is commonly seen in cardiovascular surgery, activates inflammation and causes renal damage. In this experimental study, we aimed to assess the effects of different doses insert ignore into journalissuearticles values(5 and 30 mg/kg); of methylprednisolone insert ignore into journalissuearticles values(MP);, which has anti-inflammatory effect, on renal ischemia/reperfusion insert ignore into journalissuearticles values(I/R); injury. Methods: Thirty-two male Wistar albino rats were randomly divided into four groups insert ignore into journalissuearticles values(n = 8);. The sham group underwent midline laparotomy and dissection of the abdominal aorta without occlusion while the I/R group underwent suprarenal aortic ischemia for 45 minutes followed by 180 minutes of reperfusion. In the 5 mg/kg MP and 30 mg/kg MP groups, MP was administered intraperitoneally. At the end of the experiment, blood samples were obtained, and kidneys were extracted. Results: Pretreatment with methylprednisolone did not influence serum BUN and creatinine levels. Serum TNF-α levels and ischemia-modified albumin levels were significantly lower in the MP groups compared to the I/R group insert ignore into journalissuearticles values( p < 0.05);. Histological examination demonstrated severe injury in the I/R group and treatment with MP attenuated the severity. The difference was significant in doses of 30 mg/kg MP. Conclusions: This results of the model of renal I/R injury presented in this work reveal the anti-inflammatory and the protective effects of MP in cases of renal I/R.
Keywords : Ischemia reperfusion injury, kidney, methylprednisolone, inflammation