The Comparison of Sensitivity of Various Methods in The Detection of Olive Tree Viruses

Authors : Serpil Erilmez

Pages : 1-13

View : 19 | Download : 12

Publication Date : 2016-09-30

Article Type : Research Paper

Abstract :To compare the sensivity of different methods used to detect the agents of viral diseases in olive trees, totaly 375 samples of leaves, fruits and flowers were collected from olive trees in Aydın, Balikesir and Izmir provinces and for further studies, 27 samples were selected from 375 ones as a one representative sample from each of counties in these provinces. The existence of 9 viral agents in these samples was checked by RT-PCR while the detection of 4 viruses was done with DAS-ELISA. When the juice from the samples was inoculated to certain test plants, they did not produce any symptoms. It was found that all samples were infected with the any of viruses entitled Arabis mosaic virus insert ignore into journalissuearticles values(ArMV);, Cherry leafroll virus insert ignore into journalissuearticles values(CLRV);, Strawberry latent ringspot virus insert ignore into journalissuearticles values(SLRSV); and Cucumis mosaic virus insert ignore into journalissuearticles values(CMV); by RT-PCR while ArMV was present in only 5 samples in DAS-ELISA. Moreover, all samples in this study were free from Olive latent-1 virus insert ignore into journalissuearticles values(OLV-1);, Olive latent-2 virus insert ignore into journalissuearticles values(OLV-2);, Olive latent-3 virus insert ignore into journalissuearticles values(OLV-3);, Olive latent ringspot virus insert ignore into journalissuearticles values(OLRSV); and Olive leaf yellowing-associated virus insert ignore into journalissuearticles values(OLYaV); by RT-PCR. The results of RT-PCR showed that the samples from 3 trees having virus-like symptoms were infected with a virus and on the contrary, 24 samples from the trees which were determined to be infected with viruses did not demonstrated any virus symptoms. In conclusion, it was concluded that RT-PCR was the most sensitive method in the detection of the viruses in olive trees at this moment and has the potential usage on the applications of quarantine and certification programs in safety.
Keywords : Olive, Virus