A Novel Technique for The Recovery, Isolation and Preliminary Evaluation of Rhizoctonia solani Mycoparasites from Soil

Authors : Hadi AYDIN, Gülay TURHAN

Pages : 43-51

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Publication Date : 2017-11-14

Article Type : Research Paper

Abstract :This paper describes a novel technique that allows detecting and isolating of R. solani mycoparasites subsist in soil incorporating the precolonized agar method and a modified nutrient medium supplemented with tolclophos-methyl . A great number of potential biocontrol agents like Trichoderma and Gliocladium could be isolated into pure culture. Most of the isolates examined macroscopically and microscopically in dual cultures had been recognized as myco parasites. By using this novel technique, various mycoparasitic isolates were obtained from soil. The majority of the isolates were in the genera of Trichoderma and Gliocladium . Fourteen Trichoderma species, Gliocladium roseum Bainier, Chaetomium sp., Geotrichum sp., Paecilomyces sp., Papulospora sp. and a Spicaria sp. were determined as having mycoparasitic activity against R. solani as well. Some of them possessed both parasitic action and antifungal antibiotic activity. This novel technique proved to be useful and showed promise for studies of the ecology of biocontrol agents added to soil or seed.
Keywords : Isolation technique, mycoparasite, Rhizoctonia solani, Trichoderma, Gliocladium