COMPOSITION ANALYSIS OF E-LIQUIDS AND THEIR EFFECTS ON HEALTHY LIVER AND PHARYNGEAL CARCINOMA CELL LINES

Authors : Caner GEYİK, Z Pinar GUMUS, Gorkem YARARBAS

Pages : 21-29

Doi:10.23902/trkjnat.1135237

View : 21 | Download : 11

Publication Date : 2023-04-15

Article Type : Research Paper

Abstract :Electronic cigarettes have become popular worldwide in recent years although their effects on human health are still not properly known. The lack of regulations brings a problem of inconsistency between ingredients and the product label. We aimed to analyse the contents of widely used e-liquids and their effects on two different cell lines. Eleven e-liquid samples were selected according to their availability and popularity. Nicotine, propylene glycol insert ignore into journalissuearticles values(PG);, glycerine insert ignore into journalissuearticles values(GLY);, and volatile compounds in e-liquids were analysed by High-Performance Liquid Chromatography insert ignore into journalissuearticles values(HPLC); and Gas Chromatography insert ignore into journalissuearticles values(GC);. 3-insert ignore into journalissuearticles values(4,5-dimethylthiazolyl-2);-2,5-diphenyltetrazolium bromide insert ignore into journalissuearticles values(MTT); cell viability assay was used to determine the effects of e-liquids on transformed human normal liver epithelial cell line insert ignore into journalissuearticles values(THLE-2); and human pharyngeal carcinoma cell line insert ignore into journalissuearticles values(Detroit 562);. Nicotine amounts were found to be consistent with product labels. and GLY were not only different between brands but also for products within the same brand. THLE-2 cell viability was inversely correlated with e-liquid concentration. However, decreases in cell viability were not correlated with nicotine amount. Interestingly, effects of several samples on Detroit 562 cells were triphasic; decrease in viability at lower doses, cell survival in mid-concentrations and loss of viability in highest doses. The analytical composition of e-liquids differs greatly among products which corresponds to different cellular effects. Viability of cancer cells does not change in a dose-dependent manner, which suggest that cellular differences may play role in the outcome of these products. 
Keywords : Electronic cigarettes, electronic liquids, chromatographic analysis, cell viability