INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS

Authors : Tuğba Nurcan YÜKSEL, Muhammed YAYLA, Duygu KÖSE, Rüstem Anıl UĞAN, Erdem TOKTAY, Pinar AKSU KILIÇLE, Elif ÇADIRCI, Zekai HALICI

Pages : 11-20

Doi:10.23902/trkjnat.1145462

View : 37 | Download : 12

Publication Date : 2023-04-15

Article Type : Research Paper

Abstract :Pomegranate peel contains bioactive ingredients such as flavonoids, ellagitannins, phenolics and proanthocyanidin compounds with high antioxidant activity. Pomegranate peel has antiapoptotic, antioxidant and anti-inflammatory effects due to its high punicalagin content. We aimed to determine the effect of pomegranate peel extract insert ignore into journalissuearticles values(PPE); on lipopolysaccharide insert ignore into journalissuearticles values(LPS);-induced uveitis. Sixty rats were seperated randomly into twelve groups insert ignore into journalissuearticles values(n = 5);. The healthy group received intraperitoneal normal saline, the uveitis group received 200 μg/kg LPS, the dexamethasone insert ignore into journalissuearticles values(DEX); group received 200 μg/kg LPS plus 1 mg/kg DEX, the PPE100, PPE300 and PPE500 groups received 200 μg/kg LPS plus 100, 300 and 500 mg/kg PPE, respectively. The eye tissues were collected at 3rd and 24th hour. and investigated molecularly insert ignore into journalissuearticles values(Relative quantification of gene expression);, biochemically insert ignore into journalissuearticles values(Superoxide dismutase activity, Glutathione and Malondialdehyde levels); and histopathologically insert ignore into journalissuearticles values(staining with Harris Hematoxylin and Eosin Y);. Tumor Necrosis Factor-α, vascular endothelial growth factor, and Caspase-3 levels markedly decreased in a dose-dependent manner in the uveitic rats following PPE administration. PPE administration significantly ameliorated uveitic disorders in oxidative stress factors including Glutathione, Superoxide dismutase and Malondialdehyde, with its effects raising in a dose-dependent manner. PPE eliminated histopathological changes in eye tissues due to uveitis. PPE can be a promising agent by contributing to alternative preventive treatment methods for uveitis with its anti-inflammatory, antioxidative, antiapoptotic and antiangiogenic effects.
Keywords : Anti inflammatory, Antioxidant, LPS induced uveitis, Pomegranate peel, Punicalagin, TNF α

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