Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr.

Authors : Tian LI, ZhenYing PENG, YuPing BI, ZhongXue FAN

Pages : 517-526

Doi:10.3906/sag-1210-99

View : 20 | Download : 10

Publication Date : 2013-10-01

Article Type : Research Paper

Abstract :V-myb avian myeloblastosis viral oncogene homolog insert ignore into journalissuearticles values(MYB); transcription factors play important roles in the processes of plant growth, development, and stress responses. In this study, a full-length cDNA sequence of a MYB gene, designated FvMYB1, was isolated from Arizona ash insert ignore into journalissuearticles values(Fraxinus velutina Torr.); for the first time. Sequence analysis showed that the deduced amino acid sequence of FvMYB1 encoded novel MYB proteins with single DNA binding domains. Based on the R3 domain amino acid sequence, the FvMYB1 was closely related to some proteins whose functions were known. The expression pattern of FvMYB1 in different organs of Arizona ash was analyzed by semiquantitative RT-PCR and real-time PCR insert ignore into journalissuearticles values(qRT-PCR);. Results showed that this gene was widely distributed in all the tested organs and the expression level of FvMYB1 was the highest in stems and the lowest in roots. The gene expression level decreased dramatically in roots under salt treatment insert ignore into journalissuearticles values(300 mM, 24 h);, while no obvious change was observed in stems. Subcellular localization indicated that FvMYB1 was localized in the nucleus. This study will lead to further research in resistance to abiotic stress in Arizona ash.
Keywords : Key words Arizona ash, expression analysis, molecular cloning, salt stress, V myb avian myeloblastosis viral oncogene homolog