Assessment of community level physiological profiles and molecular diversity of soil bacteria under different cropping systems

Authors : Biswapriya DAS, Kalyan CHAKRABARTI

Pages : 468-474

Doi:10.3906/tar-1209-52

View : 18 | Download : 9

Publication Date : 2013-08-01

Article Type : Research Paper

Abstract :Community level physiological profiles insert ignore into journalissuearticles values(CLPP); and molecular diversity of bacteria in soil under rice–rice insert ignore into journalissuearticles values(RR);, rice-fallow insert ignore into journalissuearticles values(RF);, rice-wheat insert ignore into journalissuearticles values(RW);, legume insert ignore into journalissuearticles values(LG);, mango orchard insert ignore into journalissuearticles values(MO);, and grass land insert ignore into journalissuearticles values(GL); cropping systems occurring in South West Bengal, India insert ignore into journalissuearticles values(22°22`N latitude and 86°26`E longitude); were studied. The soils were mostly acidic insert ignore into journalissuearticles values(pH 5.4 to 6.85);. The GL soil recorded the highest organic carbon insert ignore into journalissuearticles values(20.23 g kg-1); and total nitrogen insert ignore into journalissuearticles values(1.96 g kg-1);. The RF soil was the most acidic and had the poorest nutrient contents. The CLPP, as studied by carbon source utilization in BIOLOG Ecoplates, revealed that the bacteria in soils under different cropping systems could differentially utilize all the groups of carbon sources viz. carbohydrate, amino acid, carboxylic acid, polymer, amine/amide, and phenolic compound. Carbohydrate was most utilized and amine/amide and phenolic compound were least utilized. Bacterial communities in RF soil utilized the highest amount of carbohydrate and carboxylic acid and also utilized a balanced amount of other individual carbon substrates. Molecular diversity was studied by polymerase chain reaction followed by amplified ribosomal DNA restriction analysis insert ignore into journalissuearticles values(ARDRA); of soil DNA. Variations in ARDRA banding pattern followed by cluster analysis and the resulting dendrogram indicated that the cropping systems induced changes in soil bacterial communities. The grouping of uncultivated soils insert ignore into journalissuearticles values(MO and GL); in a separate cluster clearly indicated the presence of different bacterial communities.
Keywords : Key words Amplified ribosomal DNA restriction analysis, BIOLOG, cluster analysis, community level physiological profile, cropping system, microbial communities