Production of cellulase by Pleurotus ostreatus and Pleurotus sajor-caju in solid state fermentation of lignocellulosic biomass

Authors : Md Ibrahim KHALIL, Mia Mahmudul HOQUE, Mafroz Ahmed BASUNIA, Nadia ALAM

Pages : 333-341

Doi:10.3906/sag-1206-77

View : 32 | Download : 12

Publication Date : 2011-08-01

Article Type : Research Paper

Abstract :Bioconversion of cellulosic components into fermentable sugars has currently been accomplished with the help of the microbial enzyme, cellulase. In this study, Pleurotus ostreatus insert ignore into journalissuearticles values(Jacquin ex Fr.); Kummer and Pleurotus sajor-caju insert ignore into journalissuearticles values(Fr.); Singer were tested for cellulase production by solid state fermentation insert ignore into journalissuearticles values(SSF); using saw dust, sugarcane bagasse, and paddy straw as substrate. To enhance production, the substrates were pretreated with 1% and 4% NaOH solutions. Supernatants of extracted enzyme solutions were used to estimate reducing sugar and soluble protein, as well as enzyme activities. Maximum production of reducing sugar and soluble protein were recorded in 12 and 10 day fermented products, respectively. In the production of reducing sugars, P. ostreatus insert ignore into journalissuearticles values(42.8 mg g-1); was found to be more efficient than P. sajor-caju insert ignore into journalissuearticles values(10.9 mg g-1);, while P. sajor-caju was more efficient in soluble protein production insert ignore into journalissuearticles values(40.2 mg g-1); compared to P. ostreatus insert ignore into journalissuearticles values(3.1 mg g-1);. P. ostreatus was found to be more efficient in the individual components of cellulase enzyme production than P. sajor-caju. All the enzyme activities were found to attain a peak at 10 days of fermentation. The highest activities of endoglucanase, exoglucanase, and b-glucosidase were recorded as 7.08, 7.36, and 3.60 units insert ignore into journalissuearticles values(µmole of glucose released/min/g substrate);, respectively, for P. ostreatus, while that of P. sajor-caju were 1.9, 2.03, and 2.63 units, respectively. Total cellulase activity was found to reach a maximum at 10 days of fermentation for both strains. For P. ostreatus it was recorded as 3.51 units, while for P. sajor-caju it was 0.82 units. This study suggests P. ostreatus as an important source of cellulase enzymes.
Keywords : Key words Cellulase, Pleurotus ostreatus, Pleurotus sajor caju, solid state fermentation