Regeneration and Histological Analysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.)

Authors : Yeşim YALÇIN MENDİ, Muzaffer İPEK, Nihal BUZKAN, Yıldız AKA KAÇAR, Sebahattin ÇÜRÜK

Pages : 165-172

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Publication Date : 2009-04-01

Article Type : Research Paper

Abstract :Two different types of explant insert ignore into journalissuearticles values(proximal and flamingo-bill); from Emphasis seedlings, a hybrid cultivar of bottle gourd insert ignore into journalissuearticles values(Lagenaria siceraria insert ignore into journalissuearticles values(Molina); Stand.);, germinated under dark and light conditions were cultured on 9 regeneration MS media containing various combinations of BA insert ignore into journalissuearticles values(0, 1.0, and 2.0 mg l-1); and IAA insert ignore into journalissuearticles values(0, 0.25, and 0.5 mg l-1);. Comparison of the explant types showed that the flamingo-bill type explant had better shoot formation than did the proximal explant. The MS medium containing 1 mg l-1 of BA was optimal for shoot formation capacity when flamingo-bill type explants germinated in the dark insert ignore into journalissuearticles values(44%); and light insert ignore into journalissuearticles values(36%); were used. Histological analysis showed that explant cell division began after 3 days in regeneration medium and formation of primordium was observed in the tissues in culture between days 5 and 7. Differentiation of meristimatic structures was first observed after 9 days and development completed after 9-12 days in the culture.
Keywords : Bottle Gourd, Lagenaria siceraria, regeneration, histology, explant type