Rapid Characterization of Garlic Clones with Locus-Specific DNA Markers

Authors : Meryem İPEK, Ahmet İPEK, Philipp W SIMON

Pages : 357-362

View : 20 | Download : 9

Publication Date : 2008-09-01

Article Type : Research Paper

Abstract :Maintenance of redundant garlic insert ignore into journalissuearticles values(Allium sativum L.); accessions is expensive due to the necessity of yearly regenerating garlic accessions in germplasm centers. Therefore, rapid characterization of garlic accessions is important for avoiding duplicated genotypes. For this purpose we developed several locus-specific polymerase chain reaction insert ignore into journalissuearticles values(PCR);-based DNA markers, and tested them for the characterization of garlic clones that were previously analyzed using amplified fragment length polymorphism insert ignore into journalissuearticles values(AFLP); and randomly amplified polymorphic DNA insert ignore into journalissuearticles values(RAPD); markers. Among 25 garlic clones, 4 locus-specific markers generated 24 polymorphic alleles. Garlic accessions within a group determined by AFLP and RAPD analyses generally had the same alleles as the locus-specific markers. In addition, several alleles of these locus-specific markers were associated only with certain AFLP and RAPD groups, which can allow rapid assessment of newly collected garlic accessions; therefore, these locus-specific markers can be used as another tool for the rapid characterization of garlic germplasm collections.
Keywords : Allium sativum L, genetic characterization, DNA markers, germplasm