Antioxidant Activity of Cauliflower (Brassica oleracea L.)

Authors : Ekrem KÖKSAL, İlhami GÜLÇİN

Pages : 65-78

View : 20 | Download : 11

Publication Date : 2008-01-01

Article Type : Research Paper

Abstract :Recently, a number of studies on the health benefits associated with fruits, vegetables, herbs and spices demonstrated that they possess potent antioxidant, anti-inflammatory, anti-mutagenic, and anti-carcinogenic activity. The potential antioxidant activity of water and ethanol extracts of cauliflower insert ignore into journalissuearticles values(Brassica oleracea L.); were investigated to evaluate their potential value as a natural ingredient for foods or cosmetic application. In this study antioxidant activity was measured by 2,2`-azino-bisinsert ignore into journalissuearticles values(3-ethylbenzthiazoline-6-sulfonic acid); insert ignore into journalissuearticles values(ABTS); radical scavenging, 1,1-diphenyl-2-picryl-hydrazyl free radical insert ignore into journalissuearticles values(DPPH.); scavenging, N,N-dimethyl-p-phenylenediamine dihydrochloride insert ignore into journalissuearticles values(DMPD); radical scavenging, superoxide anion insert ignore into journalissuearticles values(O2.-); radical scavenging, total antioxidant activity, reducing activity using Fe+3-Fe+2 transformation and CUPRAC assays, hydrogen peroxide insert ignore into journalissuearticles values(H2O2); scavenging, and ferrous metal chelating activity assays. The water extract of cauliflower insert ignore into journalissuearticles values(WEC); and ethanol extract of cauliflower insert ignore into journalissuearticles values(EEC);, as antioxidants, neutralized the activity of radicals and inhibited the peroxidation reactions of linoleic acid emulsion. Total antioxidant activity was measured according to the ferric thiocyanate method. a-Tocopherol and trolox, a water-soluble analogue of tocopherol, were used as the reference antioxidant compounds. WEC and EEC showed 88.6% and 80.1% inhibition of lipid peroxidation of linoleic acid emulsion, respectively, at the concentration of 30 µg ml-1. On the other hand, at the same concentration, the standard antioxidants a-tocopherol and trolox exhibited 68.1.4% and 81.3% inhibition of peroxidation of linoleic acid emulsion, respectively. In addition, WEC and EEC had effective DPPH., ABTS.+, DMPD.+, and superoxide anion radical scavenging, hydrogen peroxide scavenging, total reducing power, and metal chelating of ferrous ion activity. Also, those various antioxidant activities were compared to a-tocopherol and trolox as references antioxidants.
Keywords : Cauliflower, Brassica oleracea, antioxidant activity, radical scavenging