Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers

Authors : Zübeyir DEVRAN

Pages : 337-341

View : 17 | Download : 8

Publication Date : 2002-12-01

Article Type : Research Paper

Abstract :Fragments containing the internal transcribed spacer insert ignore into journalissuearticles values(ITS); ribosomal rDNA were amplified from Meloidogyne incognita, M. javanica, M. arenaria and M. hapla by polymerase chain reaction insert ignore into journalissuearticles values(PCR);. Only digestion of the ITS region with the enzyme RsaI significantly distinguished M. hapla from other species, while other enzymes, EcoRI and BamHI, were not effective in distinguishing these nematode species. PCR was also done with mitocondrial DNA insert ignore into journalissuearticles values(mtDNA); markers for species discrimination. The obtained amplification product of 600 bp was digested with HinfI restriction endonuclease allowing discrimination among the four root-knot species. mtDNA was found to be a useful tool for discriminating between the important Meloidogyne species in this study.
Keywords : Root nematodes, rDNA, mtDNA, diagnostics