Effects of Genotype and Concentration of 2,4-D on Callus Induction and Plant Regeneration from Young inflorescences of Dallisgrass ( Paspalum dilatatum Poir)

Authors : Ersin CAN

Pages : 113-120

View : 24 | Download : 11

Publication Date : 2000-02-01

Article Type : Research Paper

Abstract :This study was conducted to determine the effects of genotype and 2,4-D concentrations on the callus induction and plant regeneration from young inflorescences of dallisgrass insert ignore into journalissuearticles values( Paspalum dilatatumPoir);. Segments of young inflorescences from six different ecotypes of dallisgrass were cultured on MS-medium containing different con-centrations of 2,4-D insert ignore into journalissuearticles values( 2, 4, 6, 8 and 10 mg/l);. The results of the study showed that the ecotypes were significantly different in cal-lus induction ratio, callus weight per petri dish and plant regeneration from the young inflorescences. With respect to the ecotypes, callus induction ratio varied from 17.5 % to 65 %, callus weight from 75.25 to 365.1 mg/petri dish and number of regenerates per inflorescence segment from 0.775 to 1.612. The callus induction ratio, callus weight and regeneration ratio were also signifi-cantly influenced by the 2,4-D concentrations. The segments cultured on the MS medium containing 6 mg/l of 2,4-D gave the high-est values of callus induction ratio insert ignore into journalissuearticles values(74 %);, callus weight insert ignore into journalissuearticles values(369.3 mg/petri dish); and regeneration ratio insert ignore into journalissuearticles values(2.094 regenerates per seg-ment);.
Keywords : Turk J Agric For, 24, 2000, 113 120 Turk J Agric For, vol 24, iss 1