Somatic embryogenesis and encapsulation of immature bulblets of an ornamental species, grape hyacinths (Muscari armeniacum Leichtlin ex Baker)

Authors : Bahtiyar Buhara YÜCESAN, Feride ÇİÇEK, Ekrem GÜREL

Pages : 716-722

Doi:10.3906/tar-1308-81

View : 25 | Download : 11

Publication Date : 2014-10-01

Article Type : Research Paper

Abstract :An efficient in vitro regeneration system was developed for the ornamental Muscari armeniacum on Linsmaier and Skoog basal medium insert ignore into journalissuearticles values(LS); supplemented with benzyladenine insert ignore into journalissuearticles values(BA); at varying concentrations insert ignore into journalissuearticles values(0.5, 1.0, or 2.0 mg/L); alone or in combination with 0.5 mg/L alpha-naphthalene acetic acid insert ignore into journalissuearticles values(NAA);. The highest mean number of direct somatic embryo formations was observed on LS medium containing 2.0 mg/L BA and 0.5 mg/L NAA, with a mean of 7.9 somatic embryos per explant after 10 weeks of culture. Green nodular calli induced on LS medium containing 5.0 mg/L BA alone or in combination with 0.5 mg/L NAA were transferred to LS medium supplemented with or without 0.5 mg/L gibberellic acid insert ignore into journalissuearticles values(GA3); for 8 weeks, producing 23.3 immature bulblets. Immature bulblets produced in vitro were either embedded in a sodium alginate matrix for the encapsulation process or were transferred directly to LS medium supplemented with or without GA3 at 0.5 or 1.0 mg/L for growth and development for 6 weeks. Encapsulated bulblets were then stored at 4 °C in darkness for 10 weeks and almost all encapsulated bulblets retained their viability and resumed their growth under nonaxenic greenhouse conditions.
Keywords : Encapsulation, Muscari armeniacum, ornamental plants, somatic embryogenesis