Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) accessions through retrotransposon-based interprimer binding sites (iPBSs) markers

Authors : SEDA NEMLİ, TALA KIANOOSH, MUHAMMED BAHATTİN TANYOLAÇ

Pages : 940-948

View : 36 | Download : 12

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :The common bean insert ignore into journalissuearticles values(Phaseolus vulgaris L.); is an important crop throughout the world and is highly recommended for many developing countries due to its nutritional components. A retrotransposon-based DNA marker system, interprimer binding sites insert ignore into journalissuearticles values(iPBSs);, developed from reverse transcriptase primer binding sites, was used to determine genetic diversity in common bean accessions. The genetic polymorphism and effectiveness of the marker system were evaluated. A total of 180 polymorphic bands were detected using 47 iPBS primers among 67 common bean accessions, with an average of 4 polymorphic fragments per primer. The genetic similarity between accessions was calculated using the software NTSYS-pc and ranged from 0.09 to 0.99. The average polymorphism information content value for the iPBS markers was 0.73. A model-based clustering method classified the common bean accessions into 4 populations using STRUCTURE software. The results indicated that the iPBS marker method can successfully determine the genetic diversity level in common bean accessions.
Keywords : Common bean, retrotransposon, iPBS, population structure, genetic diversity