Pokeweed (Phytolacca americana L.) antiviral protein inhibits Zucchini yellow mosaic virus infection in a dose-dependent manner in squash plants

Authors : Hikmet Murat SİPAHİOĞLU, İlhan KAYA, Mfa USTA, Murat ÜNAL, Dilek ÖZCAN, Meryem Özer DİLMEN, Abdullah GÜLLER, Vicente PALLAS

Pages : 256-262

View : 26 | Download : 7

Publication Date : 2017-08-01

Article Type : Research Paper

Abstract :Pokeweed antiviral protein insert ignore into journalissuearticles values(PAP); of Phytolacca americana L. insert ignore into journalissuearticles values(pokeweed); is a single-chain ribosome-inactivating protein insert ignore into journalissuearticles values(RIP); characterized by its ability to depurinate plant ribosomes. Here, we isolated, cloned, and expressed the ribosome inactivating protein insert ignore into journalissuearticles values(RIP); gene, designated as pokeweed antiviral protein type 1 insert ignore into journalissuearticles values(PAP I);, from the summer leaves of pokeweed collected from the Black Sea region insert ignore into journalissuearticles values(Turkey);. Our findings presented here provide direct evidence that exogenous application of PAP I causes concentration-dependent inhibition of Zucchini yellow mosaic virus insert ignore into journalissuearticles values(ZYMV); infection on squash plants. Squash plants were exposed to PAP I protein with and without DMSO for four consecutive days. Regular spraying of approximately 30 kDa recombinant PAP I at 2 µg mL?1 concentration prevented treated plants from mechanical virus infection. PAP I showed antiviral activity in 9 plants out of 15 inoculated plants. Remarkably, simultaneous application of PAP, DMSO, and ZYMV did not prevent virus infection, suggesting that PAP did not have any effect on viral RNA. In the absence of ZYMV the purified peptide was not cytotoxic for squash plants, although a reduction of plant size, possibly caused by host ribosome depurination, was observed.
Keywords : Phytolacca americana, antiviral protein, expression, inhibition, Zucchini yellow mosaic virus, squash