In vitro production protocol of Vaccinium uliginosum L. (bog bilberry) growing in the Turkish flora

Authors : MUSTAFA CÜCE, ATALAY SÖKMEN

Pages : 294-304

View : 33 | Download : 11

Publication Date : 2017-08-01

Article Type : Research Paper

Abstract :This novel approach was designed to increase the production capacity of Vaccinium uliginosum L. via direct organogenesis. Lateral buds containing one or two leaves were initially cultured in McCown woody plant medium insert ignore into journalissuearticles values(WPM);, Anderson`s rhododendron medium, and Murashige and Skoog basal media, each supplemented with zeatin/indole-3-butyric acid insert ignore into journalissuearticles values(IBA); and zeatin/naphthalene acetic acid insert ignore into journalissuearticles values(NAA); insert ignore into journalissuearticles values(1.0/0.1 mg L-1);. WPM containing the zeatin/IBA combination was the most effective as the basal medium. Various plant growth regulators insert ignore into journalissuearticles values(PGRs); were then investigated for the best shoot multiplication, and zeatin was found to be most favorable PGR in all cases. The highest shoot length and shoot number at 40.02 mm and 3.73-fold were obtained from the medium supplemented with 2.0/0.1/0.2 mg L-1 zeatin/IBA/gibberellic acid. Rooting capability was also studied by using WPM with IBA, indole-3-acetic acid, and NAA insert ignore into journalissuearticles values(0.25-1.0 mg L-1); with or without activated charcoal insert ignore into journalissuearticles values(AC);. It was found that 0.5/1.0 mg L-1 IBA/AC gave the highest rooting percentage at 18%. Rooted plantlets transplanted into peat:perlite insert ignore into journalissuearticles values(2:1); substrates were subsequently acclimatized under climate chamber conditions.
Keywords : Indole 3 butyric acid, micropropagation, 2iP, Vaccinium uliginosum, sucrose, zeatin