Comparison of extraction methods optimised by RSM for extraction of some bioactives from liquorice samples

Authors : Fahriye Şeyma ÖZCAN, Nihat ÖZCAN, Ahmet Abdullah US, Osman SAĞDIÇ

Pages : 24-38

View : 18 | Download : 11

Publication Date : 2020-02-01

Article Type : Research Paper

Abstract :This study compared the extraction efficiency of 3 different techniques, ultrasound-assisted extraction insert ignore into journalissuearticles values(UAE);, microwaveassisted extraction insert ignore into journalissuearticles values(MAE);, and the classical extraction method, in obtaining some bioactive and allergen compounds, namely liquiritin insert ignore into journalissuearticles values(LQ);, glycyrrhizin insert ignore into journalissuearticles values(GL);, glycyrrhetinic acid insert ignore into journalissuearticles values(GA);, and carbenoxolone insert ignore into journalissuearticles values(CBX);, from liquorice samples of Glycyrrhiza glabra grown in Turkey. Extraction parameters for each technique were optimised by the response surface methodology-central composite design approach. Concentrations of LQ, GL, GA, and CBX, which were selected as the responses of selected independent variables, were determined by liquid chromatography-electrospray ionisation tandem mass spectrometry. One-way variance analysis was performed to evaluate the significance of the results. MAE was revealed as the most effective technique for extracting LQ insert ignore into journalissuearticles values(mean: 8.52 mg g-1);, GL insert ignore into journalissuearticles values(mean: 76.65 mg g-1);, and GA insert ignore into journalissuearticles values(mean: 1138.84 μg g-1); from liquorice, while the classical technique was the most effective for obtaining CBX insert ignore into journalissuearticles values(mean: 5.75 μg g-1);.
Keywords : LC ESI MS MS, liquorice, microwave, response surface methodology ultrasound