Changes in the biochemical compounds of Vaccinium myrtillus, Vaccinium vitis-idaea, and forest litter collected from various forest types

Authors : Vilma KASKONİENE, Kristina BİMBİRAİTESURVİLİENE, Nicola TİSO, Laima CESONİENE, Remigijus DAUBARAS, Audrius Sigitas MARUSKA

Pages : 557-566

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Publication Date : 2020-12-01

Article Type : Research Paper

Abstract :The aim of this study was to evaluate the impact of forest clear-cutting on the phenolic compounds and antiradical activity of Vaccinium myrtillus, Vaccinium vitis-idaea, and forest litter collected during the vegetation stages in 2 forests types insert ignore into journalissuearticles values(Pinetum vacciniosum and Pinetum vaccinio-myrtillosum);. The Folin-Ciocalteu method, aluminum trichloride colorimetric assay, and 2,2-diphenyl-1-picryihydrazyl insert ignore into journalissuearticles values(DPP11); test were applied to perform the total phenolic content insert ignore into journalissuearticles values(TPC);, total flavonoid content insert ignore into journalissuearticles values(TFC);, and antiradical activity analyses of the extracts of the underground and aboveground parts of the plants and forest litter. The TPC content varied from 35.87 to 229.76 mg/g insert ignore into journalissuearticles values(expressed as rutin equivalents); and 120.03 to 309.64 mg/g in the aboveground extracts of V. myrtillus and V. viiis-idaea, respectively. Remarkably lower amounts of phenolic compounds were determined in the underground extracts of the tested shrubs. The TPC content in the forest litter ranged from 3.12 to 11.89 mg/g. The radical scavenging activity insert ignore into journalissuearticles values(RSA); varied from 3.12 to 434.68 mg/g; the lowest antiradical activity was determined in the underground extracts, while the highest was in the aboveground extracts of V myrtillus. The TFC was dependent on the vegetation phase, forest type, and clear-cutting, and varied from 7.97 to 40.18 mg/g in the aboveground extracts of the tested plants. Flavonoids were not detected in the underground extracts of the samples or in the forest litter. The chemometric analysis revealed statistically significant trends of environmental impact on V myrtillus and V vitis-idaea at the different vegetation stages. Hypotheses testing showed that the TPC, TFC and RSA expressed statistically significant insert ignore into journalissuearticles values(alpha <= 0.05); changes in 68%, 60% and 71% of the tested samples after clear-cutting, respectively.
Keywords : Bilberry, chemometric methods, lingonberry, total phenolic content, total flavonoid content, vegetation phase