Vigor difference during storage and germination in Indian mustard explained by reactive oxygen species and antioxidant enzymes

Authors : Nipa BİSWAS, Sangita YADAV, Shiv Kumar YADAV, Ravish CHOUDHARY, Navinder SAİNİ, Anil DAHUJA

Pages : 577-588

View : 19 | Download : 8

Publication Date : 2020-12-01

Article Type : Research Paper

Abstract :Canola-type genotypes in Indian mustard insert ignore into journalissuearticles values(Brassica juncea); are a new kind of quality resource developed for their low levels of erucic acid insert ignore into journalissuearticles values(<2%); and glucosinolate insert ignore into journalissuearticles values(<30 μmole/g defatted meal); contents. Single-zero insert ignore into journalissuearticles values(low erucic acid); and double-zero insert ignore into journalissuearticles values(low erucic acid and glucosinolate content); genotypes of Indian mustard have less vigor. Conventional genotypes insert ignore into journalissuearticles values(high erucic acid and glucosinolate contents); havea significantly higher seedling vigor index-II insert ignore into journalissuearticles values(SVI-II); and single-zero genotypes have a significantly higher SVI-I, whereas double-zero genotypes have been observed to have a significantly lower SVI-I and SVI-II. To know the possible reasons for the differences in vigor, the seed quality parameters, reactive oxygen species insert ignore into journalissuearticles values(ROS); contents insert ignore into journalissuearticles values(superoxide radicals insert ignore into journalissuearticles values(O2−·); and hydrogen peroxide);, lipid peroxidation, and antioxidant enzyme activity were examined. In the dry seeds, the conventional genotypes revealed lower ROS contents and higher catalase and peroxidase enzyme activity. This trend was reversed in the double-zero genotypes, which could be the reason why they were more susceptible to oxidative damage. During seed germination, an increase in the ROS contents, and corresponding increase in antioxidant enzyme activity, was noticed, which was highest in the conventional genotypes, followed by the single-zero genotypes. Double-zero genotypes showed the lowest increase in ROS contents and antioxidant enzyme activity during this period. This meant that the required attributes were met for maintaining oxidative balance within the cells and triggering physiological activities to reach high vigor. This study proposed 2 causes for the poor vigor of the double-zero genotypes; first, in the dry seeds, the ROS remained high due to low antioxidant activity insert ignore into journalissuearticles values(ROS scavengers); and the second was less generation of O2 −· during germination.
Keywords : Canola, B juncea, seed quality, germination, antioxidant activity