Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey

Authors : Tayfun KAYA, Belma ASLIM, Ergin KARİPTAŞ

Pages : 307-317

Doi:10.3906/biy-1303-18

View : 25 | Download : 14

Publication Date : 2014-12-01

Article Type : Research Paper

Abstract :In this study, 26 Pseudomonas spp. were isolated from a stream polluted by factory waste and from petroleum-contaminated soil. The surface tension insert ignore into journalissuearticles values(ST); of the cultures was used as a criterion for the primary isolation of biosurfactant-producing bacteria. Biosurfactant production was quantified by ST reduction, critical micelle concentration insert ignore into journalissuearticles values(CMC);, emulsification capacity insert ignore into journalissuearticles values(EC);, and cell surface hydrophobicity insert ignore into journalissuearticles values(CSH);. Two of the isolates, P. aeruginosa 78 and 99, produced rhamnolipid biosurfactant. The strains started rhamnolipid production in the logarithmic phase. They decreased the ST of the culture from 73 dyne/cm2 to 29 and 33 dyne/cm2, and the CMC of produced rhamnolipids were 115 and 130 mg/L, respectively. P. aeruginosa 78 and 99 strains emulsified benzene and n-hexane at the highest rates, and the surfaces of these strains were 73% and 65% and 62% and 72% more hydrophobic for benzene and toluene, respectively.
Keywords : Key words Rhamnolipid, Pseudomonas spp, surface tension, critical micelle concentration, emulsification activity, cell surface hydrophobicity