Activity of antioxidant enzymes during induction of morphogenesis of Fritillaria meleagris in bulb scale culture

Authors : Marija PETRIC, Sladana JEVREMOVIC, Milana TRIFUNOVIC, Vojin TADIC, Snezana MILOSEVIC, Angelina SUBOTIC

Pages : 328-338

Doi:10.3906/biy-1309-45

View : 20 | Download : 9

Publication Date : 2014-12-01

Article Type : Research Paper

Abstract :We investigated the activity of 3 antioxidant enzymes: superoxide dismutase, catalase, and peroxidase during morphogenesis of Fritillaria meleagris L. in vitro in bulb scale culture. Bulb cultures of F. meleagris were established on nutrition medium supplemented with 1.0 mg L-1 thidiazuron. Bulbs were grown at standard insert ignore into journalissuearticles values(24 °C); and low temperatures insert ignore into journalissuearticles values(4 °C); or at an increased sucrose concentration insert ignore into journalissuearticles values(4.5%); during the first 6 weeks insert ignore into journalissuearticles values(pretreatments);. All bulbs were then grown for 4 weeks at standard temperature on 2, 4-dichlorophenoxyacetic acid and kinetin or thidiazuron insert ignore into journalissuearticles values(at concentrations of 1 mg L-1 each); in order to examine the influence of the above-mentioned pretreatments and nutrient medium composition on enzyme activity. Enzyme activity was measured 7, 14, 21, and 28 days after the start of morphogenesis induction. Superoxide dismutase and catalase showed the highest activity at the beginning of morphogenesis in vitro. Peroxidase activity was the highest in bulb segments immediately after isolation following pretreatments and on both nutrition media. We also determined the effect of pretreatment coupled with low temperature insert ignore into journalissuearticles values(4 °C); on growth and development of F. meleagris in vitro and chlorophyll and carotenoids content.
Keywords : Key words Snake`s head fritillary, morphogenesis, superoxide dismutase, catalase, peroxidase