Temperature-induced stress response in Lymantria dispar neurosecretory neurons

Authors : Larisa ILIJIN, Milena VLAHOVIC, Vesna Peric MATARUGA, Ivana KMETIČ, Anja GAVRILOVIĆ, Dragana MATIĆ, Marija MRDAKOVIĆ

Pages : 157-167

Doi:10.3906/biy-1306-75

View : 22 | Download : 15

Publication Date : 2014-12-01

Article Type : Research Paper

Abstract :The release of neurosecretory material from A2 neurosecretory neurons insert ignore into journalissuearticles values(NSNs); was stimulated in Lymantria dispar fourth instar caterpillars exposed to a temperature of 35 °C for 1, 12, and 24 h, as well as those allowed recover after exposure insert ignore into journalissuearticles values(12 h at 35 °C, then 12 h at 23 °C);. The levels of 2 protein forms with the same molecular mass as bombyxin insert ignore into journalissuearticles values(3-4 and 4-5 kDa); increased with prolonged exposure to 35 °C. The second band was present only in the groups exposed to this stressor. There was intensified synthetic activity and a low level of secretion in L2` NSNs after exposure to 35 °C. We previously found these NSNs to be immunopositive for prothoracicotropic neurohormone. After this stress, densitometric analysis revealed a decreased amount of the 11-12 kDa isoform insert ignore into journalissuearticles values(present in the control group);. The new isoform insert ignore into journalissuearticles values(13-15 kDa);, expressed after exposure of the insects to a high temperature, increased in amount with prolonged exposure and after recovery at 23 °C. Short-term exposure of caterpillars to high temperatures insert ignore into journalissuearticles values(35 °C); is a stressor and activates carbohydrate metabolism, while PTTH immunopositive NSNs are secretory-inactive during acute thermal stress regimes.
Keywords : Key words High temperature, gypsy moth, medial and lateral neurosecretory neurons