The genus Crocus, series Crocus (Iridaceae) in Turkey and 2 East Aegean islands: a genetic approach

Authors : Osman EROL, Hilal Betül KAYA, Levent ŞIK, Metin TUNA, Levent CAN, Muhammed Bahattin TANYOLAÇ

Pages : 48-62

Doi:10.3906/biy-1305-14

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Publication Date : 2014-12-01

Article Type : Research Paper

Abstract :In this study, a total of 26 Crocus specimens from different locations across Turkey and 2 East Aegean islands insert ignore into journalissuearticles values(Chios and Samos); were analyzed using 12 amplified fragment length polymorphism insert ignore into journalissuearticles values(AFLP); primer combinations to obtain information on genetic diversity, population structure, and genetic relationships. A total of 369 polymorphic AFLP bands were generated and scored as binary data. Genetic similarities were determined. Cluster analysis revealed 4 major groups among the 26 genotypes examined in this study. The nuclear DNA contents insert ignore into journalissuearticles values(2C); of the 26 Crocus specimens were found to range from 5.08 pg in C. asumaniae to 9.75 pg in C. sativus. Polymorphic information content insert ignore into journalissuearticles values(PIC); values were used to examine the capacity of the various primer pairs to amplify polymorphisms in the Crocus specimens. The PIC values ranged from 0.218 insert ignore into journalissuearticles values(M-CAA/E-AGC); to 0.512 insert ignore into journalissuearticles values(M-CAT/E-AAG); and showed an average of 0.34. In sum, we herein used AFLP analysis to identify a high level of polymorphism among Crocus specimens collected from various locations in Turkey and Greece, and our structural analysis yielded 2 reconstructed populations. These findings provide new insight into the relationships among different Crocus genotypes and show that AFLP analysis can be useful for Crocus diversity studies.
Keywords : Key words Amplified fragment length polymorphism, Crocus, genetic diversity, genetic structure, nuclear DNA content