Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew

Authors : Zohreh JALALPOUR, Leila SHABANI, Ladan AFGHANI, Majid SHARIFITEHRANI, SayedAsadollah AMINI

Pages : 76-82

Doi:10.3906/biy-1306-91

View : 13 | Download : 6

Publication Date : 2014-12-01

Article Type : Research Paper

Abstract :Cell suspension cultures of Taxus baccata were treated with 2 elicitor compounds, methyl jasmonate insert ignore into journalissuearticles values(MeJA); and squalestatin insert ignore into journalissuearticles values(S);, individually and in combination for 7 days to determine if they mediated the enhancement of biosynthesis of endogenous jasmonate through induction of lipoxygenase insert ignore into journalissuearticles values(LOX); activity. Total phenolic compounds, total flavonoids, total antioxidants, phenylalanine ammonia-lyase insert ignore into journalissuearticles values(PAL);, polyphenol oxidase insert ignore into journalissuearticles values(PPO);, and LOX activities in 5-month-old yew cell cultures were studied. Our results showed that MeJA and S could stimulate production of phenol derivatives in cell suspension cultures of T. baccata. In parallel to the induction of phenolic production in elicited cells, results showed that activities of PAL and PPO enzymes and total antioxidants significantly increased in Taxus cells in response to MeJA and S. Maximal activities of lipoxygenase were obtained 48 h after treatment with MeJA insert ignore into journalissuearticles values(100 µM);, S insert ignore into journalissuearticles values(0.1 µM);, and the combination of the 2 elicitors. Results showed that MeJA and S are effective elicitors for increasing phenolic production in Taxus cell suspension cultures, likely through increasing LOX activity followed by an increase in endogenous jasmonate.
Keywords : Key words Antioxidant, lipoxygenase, phenylalanine ammonia lyase, squalestatin, Taxus