In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

Authors : Satı UZUN, İskender PARMAKSIZ, Serkan URANBEY, Semra MİRİCİ, Ercüment Osman SARIHAN

Pages : 83-88

Doi:10.3906/biy-1307-52

View : 27 | Download : 8

Publication Date : 2014-12-01

Article Type : Research Paper

Abstract :An efficient in vitro bulblet production procedure from immature zygotic embryos of endemic and endangered Muscari muscarimi Medik. was described in the current study. Zygotic embryos were first isolated from immature seeds and cultured on different nutrient media compositions supplemented with various combinations of a-naphthalene acetic acid insert ignore into journalissuearticles values(NAA);, picloram, dicamba, 6-benzylaminopurine insert ignore into journalissuearticles values(BAP);, and thidiazuron insert ignore into journalissuearticles values(TDZ);. The best bulblet regeneration insert ignore into journalissuearticles values(59 bulblets per explant); was achieved in Murashige and Skoog insert ignore into journalissuearticles values(MS); medium containing 4 mg/L BAP and 0.5 mg/L NAA after 1 year of culture initiation. Regenerated bulblets were then transferred into MS medium without plant growth regulators for rooting. Bulblets produced well-developed root systems and increased their size on this medium after 2 months. All rooted bulblets were successfully transplanted into a potting mixture and acclimatized to ambient conditions.
Keywords : Key words Muscari muscarimi Medik, immature embryo, tissue culture