A comparative study of EGF effects on in vitro bovine embryo development in monoculture and sequential media

Authors : Azadeh ZAHMATKESH, Mahmood Amiri ROUDBAR, Morteza Daliri JOUPARI

Pages : 670-674

Doi:10.3906/biy-1302-74

View : 15 | Download : 10

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :Different culture system conditions can affect cleavage, zygote genome activation, embryo development, and viability. The purpose of the present study was to compare a monoculture synthetic oviductal fluid medium with modified sequential G1/G2 media and also to investigate the effect of epidermal growth factor insert ignore into journalissuearticles values(EGF); on bovine embryo development. Oocytes were aspirated from 2- to 6-mm ovarian follicles and matured in maturation media. After in vitro fertilization of matured oocytes, presumptive zygotes were cultured in test media with or without additional exogenous EGF. Results indicated that EGF significantly increased blastocyst formation but not cleavage rate in both media. There was no significant difference between the sequential and the monoculture media.
Keywords : Key words Blastocyst, cleavage, culture system, epidermal growth factor