Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant

Authors : Shamima Akhtar SHARMIN, Mohammad Jahangir ALAM, Md Mominul İslam SHEIKH, Rashed ZAMAN, Muhammad KHALEKUZZAMAN, Sanjoy Chandra MONDAL, Mohammad Anwarul HAQUE, Mohammad Firoz ALAM, İftekhar ALAM

Pages : 698-708

Doi:10.3906/biy-1212-11

View : 19 | Download : 7

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :A rapid and improved micropropagation protocol was developed for Curcuma aromatica, a threatened aromatic medicinal plant, using rhizome sprout as the explant. Stepwise optimization of different plant growth regulators, carbon sources, and basal media was adopted to establish an efficient micropropagation protocol. When cytokinins, such as benzyl amino purine insert ignore into journalissuearticles values(BAP); or 6-insert ignore into journalissuearticles values(a,a-dimethylallylamino);-purine insert ignore into journalissuearticles values(2iP);, were used either singly or in combination with naphthalene acetic acid insert ignore into journalissuearticles values(NAA); for shoot induction and multiplication, a single use of BAP was the most effective. As a carbon source, 3% insert ignore into journalissuearticles values(w/v); sucrose exhibits the greatest promotive effect on shoot initiation and proliferation compared with other carbon sources used. Among the basal media, full strength Murashige and Skoog insert ignore into journalissuearticles values(MS); media produced the best results, compared to other media studied. By using the most effective treatment from each category, an average of 13.2 shoots/per explant were produced after 6 weeks of culture. Moreover, 85% survival was achieved when rooted explants acclimatized ex vitro using a mixture of sterile sand, soil, and farmyard manure insert ignore into journalissuearticles values(1:1:1);. In addition, antimicrobial activities of rhizome extracts were evaluated. Petroleum ether and chloroform extracts of field-grown rhizome showed potential antimicrobial properties against several human pathogenic bacteria including Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella sonnei, and Shigella dysenteriae, with a minimum inhibitory concentration insert ignore into journalissuearticles values(MIC); ranging from 0.03 to 0.5 mg/mL. Thus, the optimized micropropagation protocol may offer large-scale production of plantlets to meet industrial demand for the rhizome. Moreover, our results suggest the rhizome extract of C. aromatica is a promising antimicrobial agent.
Keywords : Key words Antimicrobial activity, Curcuma aromatica, endangered, in vitro culture