Improving in vitro leaf disk regeneration system of sugarcane (Saccharum officinarum L.) with concurrent shoot/root induction from somatic embryos

Authors : Muhammad NAWAZ, İhsan ULLAH, Naeem IQBAL, Muhammad Zafar IQBAL, Muhammad Aslam JAVED

Pages : 726-732

Doi:10.3906/biy-1212-10

View : 16 | Download : 8

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :Genome engineering experiments are impeded by poor performance of regeneration systems. The present study was aimed at establishing a short and cost-effective in vitro regeneration system for elite sugarcane cultivars through simultaneous shoot/root induction. The innermost spindle leaf and shoot tip were used as explants. For callus induction, Murashige and Skoog insert ignore into journalissuearticles values(MS); medium supplemented with 2,4-dichlorophenoxyacetic acid insert ignore into journalissuearticles values(2,4-D); was used and 5.0 mg/L of 2,4-D supported maximum callus induction insert ignore into journalissuearticles values(84.5%);. Three-week-old calli were treated with different levels of benzylaminopurine insert ignore into journalissuearticles values(BAP); ranging from 0.00 to 3.5 mg/L in MS medium, where 2.5 mg/L BAP was proven to be the best level for regeneration. In a multiplication and root formation medium, 0.5 mg/L naphthalene acetic acid supported the maximum number of roots per plant. Finally, a direct somatic embryogenesis protocol was established, competent enough for simultaneous root/shoot induction. The results indicated that the plantlets were established within 12 weeks only. This in vitro regeneration protocol was fast and cost-effective and may be used for large-scale in vitro regeneration of sugarcane cultivars to save time and resources. The sugarcane cultivar SPF-234 remained the most responsive, followed by HSF-242 and CPF-246.
Keywords : Key words Sugarcane, somatic embryogenesis, fast regeneration