Evaluation of phytotoxic and mutagenic effects of some cinnamic acid derivatives using the Triticum test

Authors : Alexandra JITAREANU, Silvica PADUREANU, Gabriela TATARINGA, Cristina TUCHILUS, Ursula STANESCU

Pages : 748-756

Doi:10.3906/biy-1304-39

View : 20 | Download : 9

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :Five cinnamic acid derivatives [cinnamic acid, 2,3-dibromo-3-phenyl-propanoic acid, 2,3-dibromo-3-insert ignore into journalissuearticles values(3-bromophenyl);-propanoic acid, 2,3-dibromo-3-insert ignore into journalissuearticles values(4-hydroxy-3-methoxyphenyl);-propanoic acid, and 2,3-dibromo-3-insert ignore into journalissuearticles values(3-bromo-4-hydroxy-5-methoxyphenyl);-propanoic acid] were found to be active against Staphylococcus aureus ATCC 25923, and their minimal bactericidal concentrations were determined insert ignore into journalissuearticles values(100 µg/mL);. The first step in assessing their toxicological potential was the phytotoxicity and genotoxicity evaluation on Triticum aestivum. Wheat seeds were exposed to solutions of the tested compounds insert ignore into journalissuearticles values(100 µg/mL); for 24 and 48 h. The development of roots and seedlings, germination percentage, mitotic index, chromosomal aberrations, and total polyphenol content were analyzed. The substances caused in most experimental cases a slight inhibition in the growth of the tested plantlets in comparison to the control, with the exception of 2,3-dibromo-3-insert ignore into journalissuearticles values(3-bromo-4-hydroxy-5-methoxyphenyl);-propanoic acid insert ignore into journalissuearticles values(48 h of exposure);. All compounds inhibited the germination process and mitotic activity. No aberrant metaphases were generated, but abnormal anatelophases appeared, and 4 types of chromosomal aberrations were identified: chromosome bridges, chromosome fragments, micronuclei, and multipolar anatelophases. Wheat plantlet metabolism was also affected; the total polyphenol content decreased in the treated plantlets.
Keywords : Key words Chromosomal aberrations, micronucleus, mitotic division, phytotoxicity, polyphenols, Triticum test