Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat

Authors : Aradhika TRIPATHI, Rashmi AGGARWAL, Anita YADAV

Pages : 606-613

Doi:10.3906/biy-1301-16

View : 17 | Download : 11

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :Karnal bunt caused by Tilletia indica is a quarantine disease responsible for qualitative and quantitative losses in wheat. The present study aimed to determine the expression of defense-related genes involved in early stages of infection in response to T. indica by using the differential display reverse transcriptase-PCR method insert ignore into journalissuearticles values(DDRT-PCR); in susceptible insert ignore into journalissuearticles values(WL 711); and resistant insert ignore into journalissuearticles values(HD 29); cultivars of wheat. The DDRT-PCR banding profiles generated by the random primers in combination with oligo-dT primers of resistant and susceptible wheat plants were evaluated at 0 h, 12 h, 24 h, and 48 h after inoculation. Out of 9 sets of primers, 7 sets of primers produced remarkable induced transcripts. The highest up-regulated transcripts were observed in the resistant cultivar insert ignore into journalissuearticles values(HD 29);, whereas the down-regulated transcripts were found in the susceptible insert ignore into journalissuearticles values(WL 711); cultivar. Several sets of primers were designed from the defense-related gene sequences available in the GenBank database, and of these, 4 primer sets amplified the desired specific bands. Based on the putative functions, 3 defense-related genes, namely puroindoline protein PINB insert ignore into journalissuearticles values(AT- KB2);, \Beta-1,4-glucanase insert ignore into journalissuearticles values(AT- KB3);, and chitinase insert ignore into journalissuearticles values(AT- KB7);, and 1 housekeeping gene, actin insert ignore into journalissuearticles values(AT- KB1);, were characterized.
Keywords : Key words Defense related genes, expression, DDRT PCR, Tilletia indica, wheat