Molecular characterization of Yarrowia lipolytica strains isolated from different environments and lipase profiling

Authors : Onur AKPINAR, Füsun Bahriye UÇAR

Pages : 249-258

Doi:10.3906/biy-1201-23

View : 24 | Download : 8

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :In order to analyze yeasts that produce industrial compounds, it is essential to identify them accurately. Yarrowia lipolytica is one of the most extensively studied `nonconventional` yeasts, being a strictly aerobic microorganism capable of producing important metabolites and having an intense secretory activity, which justifies efforts to use it in industry insert ignore into journalissuearticles values(as a biocatalyst);, in molecular biology, and in studies of genetics. Therefore, in this study, an accurate identification of Y. lipolytica strains was performed using 3 different molecular biological methods insert ignore into journalissuearticles values(RFLP analysis of ITS1-5.8S rDNA-ITS2 and 18S rDNA regions and sequencing of the D1/D2 domain of the 26S rDNA region);. The 26S rRNA gene sequence of the strains showed sequence homology with various Y. lipolytica strains from the National Center for Biotechnology Information. A number of different lipids insert ignore into journalissuearticles values(tributyrin, olive oil, and fish oil); were screened in terms of the growth of Y. lipolytica strains and lipase production. It was determined that all lipid-related substrates supported lipase production levels ranging from 4.27 U/mL insert ignore into journalissuearticles values(tributyrin); to 37.08 U/mL insert ignore into journalissuearticles values(fish oil);. Fish oil insert ignore into journalissuearticles values(1%); showed maximum specific activity in the supernatant insert ignore into journalissuearticles values(264.85 U/mg of protein); and TEM TAN 46. The Y. lipolytica strain that was produced in the media containing fish oil was found to be the best lipase producer.
Keywords : Key words Yarrowia lipolytica, molecular characterization, PCR RFLP of rDNA, sequencing of D1 D2 domain, extracellular lipase