Evaluation of the genetic relationship between Fritillaria species from Turkey’s flora using fluorescent-based AFLP

Authors : Özge Karakaş METİN, Mine TÜRKTAŞ, Meral ASLAY, Erdal KAYA

Pages : 273-279

Doi:10.3906/biy-1207-62

View : 22 | Download : 13

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :In this study, the genetic relationships among 12 Fritillaria taxa collected from different regions of Turkey were investigated using the amplified fragment length polymorphism insert ignore into journalissuearticles values(AFLP); technique. Seven primer pair combinations were used for the analysis. The neighbor-joining and principal coordinate analysis methods were implemented to identify the taxonomy of the species. The analyses generated congruent results with each other and with the previous studies. The neighbor-joining analysis clustered the species into 3 groups, while the principal coordinates analysis did not support separation of the third group. The subgenera Fritillaria and Petilium diverged from each other with strong support in all analyses. The neighbor-joining tree further divided the members of subgenus Fritillaria into 2 subgroups. Statistical analysis of the data set using NTSYS 2.1 showed a very good fit between the data matrix and the cophenetic matrix for the AFLP data with an r-value of 0.91. Generating a high polymorphic band profile, this analysis presented the feasibility of using AFLP for a genetic relationship analysis of Fritillaria. This primary report on AFLP analysis of Fritillaria species could facilitate achieving a deeper understanding of genetic relationships between the species.
Keywords : Key words AFLP, Fritillaria, neighbor joining, PCoA, genetic relationships