Cr(VI) reduction by Cellulosimicrobium sp. isolated from tannery effluent

Authors : Aniqa NAEEM, Rida BATOOL, Nazia JAMIL

Pages : 315-322

Doi:10.3906/biy-1209-18

View : 21 | Download : 11

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :Crinsert ignore into journalissuearticles values(VI); is a toxic metal that has carcinogenic and mutagenic effects on all living organisms. In order to study the contribution of microbes towards Crinsert ignore into journalissuearticles values(VI); reduction into the less toxic Crinsert ignore into journalissuearticles values(III); form, an indigenous chromium-resistant bacterial strain insert ignore into journalissuearticles values(A8); was isolated from a tannery effluent. It was identified as Cellulosimicrobium sp. on the basis of morphological and biochemical characterization and 16S rRNA gene sequencing. It could tolerate up to 1800 µg mL-1 of K2CrO4 and showed an optimum reduction insert ignore into journalissuearticles values(98.6%); of Crinsert ignore into journalissuearticles values(VI); at the concentration of 900 µg mL-1 aerobically. Cell-free enzyme assay exhibited the reduction of Crinsert ignore into journalissuearticles values(VI); in vitro. A total of 4 synthetic materials, sand, PVC, stone, and rubber tubing, were used as solid support to evaluate the ability of the isolated chromium-resistant bacterial strain for biofilm formation. In column experiments among bacterial film-coated materials, sand exhibited an excellent Crinsert ignore into journalissuearticles values(VI); reduction insert ignore into journalissuearticles values(96%);, while PVC pipe, rubber tubing, and stone showed 94.5%, 90%, and 88.4% reduction potential of Crinsert ignore into journalissuearticles values(VI);, respectively, after 96 h of incubation. Fluorescent microscopy also revealed that bacterial biofilm was distributed thoroughly on the surface of the sand particles. FTIR spectroscopy showed mainly the involvement of the glycerol units, polysaccharides, and C-N insert ignore into journalissuearticles values(alkyl); functional groups of the bacterial strain insert ignore into journalissuearticles values(A8); in the reduction of Crinsert ignore into journalissuearticles values(VI);.
Keywords : Key words Biosorption, Cellulosimicrobium sp, Cr VI, reduction, FTIR spectroscopy, bacterial columns